LOCUS       Exported                3030 bp ds-DNA    circular SYN 24-6-2015
DEFINITION  Cloning vector for in vitro transcription from the SP6 promoter with
            the addition of a synthetic 30-base poly(A) tail.
ACCESSION   X65328
VERSION     .
KEYWORDS    pSP64 Poly(A)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Promega
  TITLE     Direct Submission
COMMENT     Use any MCS sites other than ApoI/EcoRI to generate a transcript 
            with a poly(A) tail.
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    7..91
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    54..83
                     /note="poly(A)"
     primer_bind     complement(100..116)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    124..140
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(148..178)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(502..1090)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1261..2121)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2122..2226)
                     /gene="bla"
                     /note="AmpR promoter"
     promoter        3014..2
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"