LOCUS       Exported                3734 bp ds-DNA    circular SYN 25-11-2013
DEFINITION  Promoterless backbone SnapFast(TM) cloning vector, on which all of 
            the other plasmids from Oxford Genetics are based.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-Core
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    18..89
                     /note="5 beta-globin insulator"
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     misc_feature    273..358
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             309..314
                     /note="Shine-Dalgarno ribosome binding site"
     misc_signal     319..325
                     /note="Kozak sequence"
     CDS             323..325
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     misc_feature    377..387
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     misc_feature    425..435
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    467..588
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     terminator      703..749
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      779..915
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    960..1031
                     /note="3 beta-globin insulator"
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(1257..1845)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             2578..2583
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             2591..3451
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3464..3600
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"