LOCUS       Exported                2070 bp ds-DNA    circular SYN 25-2-2014
DEFINITION  High copy BioBrick assembly plasmid with a chloramphenicol 
            resistance marker, for shipping parts to the Registry of Standard 
            Biological Parts.
ACCESSION   .
VERSION     .
KEYWORDS    pSB1C3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   iGEM
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     misc_feature    1..21
                     /note="BioBrick suffix"
     terminator      22..79
                     /note="his operon terminator"
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(274..862)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1045..1139
                     /gene="
                     "
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(1160..1819)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2003..2046
                     /note="bacterial terminator"
                     /note="putative bacterial transcription terminator"
     misc_feature    2049..2070
                     /note="BioBrick prefix"