LOCUS       Exported                2525 bp ds-DNA    circular SYN 08-4-2014
DEFINITION  Compact bacterial vector that employs a toxic minigene for 
            high-efficiency cloning of PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    pMiniT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        145..251
                     /gene="tnpA"
                     /note="tnpA promoter"
                     /note="promoter of the IS10 transposase"
     CDS             252..482
                     /codon_start=1
                     /product="transposase encoded by the IS10 tnpA gene"
                     /note="tnpA transposase"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTELG
                     RNLPTKARTKHNIKRIDRLLGNRHLHKERLAV"
     RBS             504..510
                     /note="Shine-Dalgarno sequence"
                     /note="ribosome binding site"
     CDS             518..523
                     /codon_start=1
                     /product="two-residue polypeptide that poisons the E. coli 
                     translation machinery"
                     /note="toxic minigene"
                     /translation="MI"
     misc_feature    524..535
                     /note="stop codons"
     promoter        565..669
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             670..1530
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1701..2289
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"