LOCUS       Exported                6749 bp ds-DNA    circular SYN 03-10-2016
DEFINITION  Vector with a hygromycin B resistance marker for transforming 
            Aspergillus species.
ACCESSION   Z32698
VERSION     .
KEYWORDS    pAN7-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CA.
  TITLE     Transformation of Aspergillus based on the hygromycin B resistance 
            marker from Escherichia coli.
  JOURNAL   Gene 1987;56:117-24.
  PUBMED    2824287
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     The trpC terminator region was corrected based on the updated 
            sequence record X02390.
FEATURES             Location/Qualifiers
     source          1..6749
                     /organism="synthetic DNA construct"
                     /lab_host="Aspergillus"
                     /mol_type="other DNA"
     promoter        1..2129
                     /gene="gpdA"
                     /note="gpdA promoter"
                     /note="promoter from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     intron          2178..2293
                     /gene="gpdA"
                     /note="gpdA intron"
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2302..3321
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     terminator      3339..4101
                     /gene="trpC"
                     /note="trpC terminator"
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     promoter        4614..4718
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             4719..5579
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5750..6338
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"