LOCUS       Exported                2814 bp ds-DNA    circular SYN 25-11-2013
DEFINITION  High copy-number E. coli vector with a multiple cloning site flanked
            by wild-type T7 promoters. For an alternative MCS, use LITMUS28i.
ACCESSION   .
VERSION     .
KEYWORDS    LITMUS38i
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Evans PD, Cook SN, Riggs PD, Noren CJ.
  TITLE     LITMUS: multipurpose cloning vectors with a novel system for 
            bidirectional in vitro transcription.
  JOURNAL   BioTechniques 1995;19:130-5.
  PUBMED    7669286
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   New England Biolabs
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
     promoter        38..142
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             143..1003
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(1045..1554)
                     /direction=LEFT
                     /note="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      1665..2253
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2350..2380
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2388..2404
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2412..2428
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2424..2786
                     /codon_start=1
                     /gene="lacZ (fragment)"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /note="lacZ-alpha"
                     /translation="MTMITPSYVIRLTIGARAIEAGWRQASLQDIWIHEFASFGRDASP
                     DVQACVDPLVKAYSESYYGLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR
                     PSQQLRSLNGEWRFAW"
     promoter        2449..2467
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2467..2579
                     /gene="lacZalpha"
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2593..2611)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2615..2631)
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"