| 载体基本信息 |
| 载体名称 |
pJYS2_crtYf |
载体抗性 |
Spectinomycin |
| 载体长度 |
4380 bp |
载体类型 |
Bacterial Expression, CRISPR |
pJYS2_crtYf 载体载体图谱
pJYS2_crtYf 载体载体序列
LOCUS Exported 4380 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Constitutive transcription of crRNA of crtYf, Spectinomycin
resistance.
ACCESSION .
VERSION .
KEYWORDS pJYS2_crtYf
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4380)
AUTHORS Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X,
Li Y, Wang R, Yang S
TITLE CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
JOURNAL Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
PUBMED 28469274
REFERENCE 2 (bases 1 to 4380)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2017-05-4"; volume: "8:15179. doi"; pages: "
10.1038/ncomms15179"
FEATURES Location/Qualifiers
source 1..4380
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 3247..3332
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3424..3451
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(3545..3562)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3716..4304)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3796..3815)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
