LOCUS       Exported                7446 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep 
            expression construct. Note that an improved version is available: 
            Addgene plasmid# 92307. See comments for details.
ACCESSION   .
VERSION     .
KEYWORDS    pAnc80L65
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, 
            Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, 
            Vandenberghe LH
  TITLE     In Silico Reconstruction of the Viral Evolutionary Lineage Yields a 
            Potent Gene Therapy Vector.
  JOURNAL   Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019.
  PUBMED    26235624
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 
            10.1016/j.celrep.2015.07.019."
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2373..2384
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(2862..2878)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2862..2878)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3023..3040)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3194..3782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3274..3293)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      4013..4040
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(4059..4874)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4782..4801
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4866..4885
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      5091..5118
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(5154..5609)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5291..5312)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5503..5522
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5736..5758
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5750..5767
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5751..5767
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5801..5817
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5802..5818
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"