LOCUS       Exported                7569 bp ds-DNA     circular SYN 23-SEP-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pEE12.4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    116..237
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(999..1587)
                     /direction=LEFT
                     /note="pEE6 ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1758..2618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2619..2723)
                     /gene="bla"
                     /note="AmpR promoter"
     terminator      2892..2919
                     /note="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     promoter        3002..3331
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      3182..3317
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             3370..4491
                     /codon_start=1
                     /note="GS"
                     /translation="MATSASSHLNKNIKQMYLCLPQGEKVQAMYIWVDGTGEGLRCKTR
                     TLDCEPKCVEELPEWNFDGSSTFQSEGSNSDMYLSPVAMFRDPFRRDPNKLVFCEVFKY
                     NRKPAETNLRHSCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYC
                     GVGADKAYGRDIVEAHYRACLYAGVKITGTNAEVMPAQWEFQIGPCEGIRMGDHLWVAR
                     FILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKHIEEAIEKLSKRH
                     RYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDR
                     RPSANCDPFAVTEAIVRTCLLNETGDEPFQYKN"
     intron          4623..4688
                     /note="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4818..4838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     polyA_signal    5263..5397
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     enhancer        5966..6345
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6346..6549
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"