Ambion's pSilencer™ adeno 1.0-CMV System combines the advantages of adenoviral vectors with an efficient siRNA expression system. With pSilencer™ adeno, you can easily deliver an siRNA expressing construct into a variety of mammalian cells and organisms.
| 载体基本信息 | |||
| 载体名称 | pSilencer adeno 1.0-CMV | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 5891 bp | 载体类型 | RNAi |
| 载体来源 | Thermo Fisher Scientific | 拷贝数 | High copy number |
| 启动子 | CMV | 克隆方法 | Enzyme Cut |
| 表达方法 | Constiutive, Stable | ||
pSilencer adeno 1.0-CMV 载体载体图谱
pSilencer adeno 1.0-CMV 载体载体序列
LOCUS Exported 5891 bp ds-DNA circular SYN 08-APR-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pSilencer adeno 1.0-CMV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5891)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5891
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 16..118
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 206..356
/note="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
enhancer 447..750
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 751..954
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
rep_origin complement(4069..4657)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4828..5688)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5689..5793)
/gene="bla"
/note="AmpR promoter"
