Expression plasmids pVITRO2-MCS are developed mainly for in vitro studies. pVITRO2-MCS allows the ubiquitous and constitutive co-expression of two genes of interest. pVITRO2-MCS plasmids carry two human ferritin composite promoters, FerH (heavy chain) and FerL (light chain). To eliminate the iron regulation, their 5’UTRs have been replaced by the 5’UTR of the mouse and chimpanzee EF-1α genes. pVITRO2-MCS plasmids are available with different selectable markers that are active both in E. coli and mammalian cells. pVITRO2-MCS plasmids contain two multiple cloning sites (MCS) for the convenient cloning of your cDNAs of interest.
| 载体基本信息 | |||
| 载体名称 | pVitro2-neo-mcs | 载体抗性 | Kanamycin |
|---|---|---|---|
| 载体长度 | 6125 bp | 载体类型 | Mammalian Expression Vectors |
| 载体来源 | Invivogen | 筛选标记 | Neomycin/G418(Geneticin) |
| 拷贝数 | High copy number | 启动子 | CMV |
| 克隆方法 | Enzyme Cut | 5引物 | EF-1a-F：TCAAGCCTCAGACAGTGGTTC |
| 表达方法 | Constiutive, Stable / Transient | ||
pVitro2-neo-mcs 载体载体图谱
pVitro2-neo-mcs 载体载体序列
LOCUS Exported 6125 bp ds-DNA circular SYN 18-SEP-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pVitro2-neo-mcs
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6125)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6125
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 9..312
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
intron 700..1644
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
polyA_signal 1728..1849
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 2037..2625
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
intron 3145..4092
/note="mEF-1-alpha intron"
/note="intron upstream of the start codon of mouse
EF-1-alpha"
misc_feature 4167..4611
/note="FMDV IRES"
/note="internal ribosome entry site (IRES) of the
foot-and-mouth disease virus"
promoter 4645..4691
/note="EM7 promoter"
/note="synthetic bacterial promoter "
promoter 4683..4701
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 4711..5505
/codon_start=1
/gene="aph(3)-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5552..6124
/note="EF-1-alpha poly(A) signal"
/note="human EF-1-alpha polyadenylation signal"
