| 载体基本信息 |
| 载体名称 |
pVAX1 |
载体抗性 |
Kanamycin |
| 载体长度 |
2999 bp |
载体类型 |
Mammalian Expression Vectors |
| 载体来源 |
Invivogen |
拷贝数 |
High copy number |
| 启动子 |
CMV |
克隆方法 |
Enzyme Cut |
| 5引物 |
CMV-F: CGCAAATGGGCGGTAGGCGTG |
3引物 |
BGH Reverse: TAGAAGGCACAGTCGAGG |
| 表达方法 |
Transient |
pVAX1 载体载体图谱
pVAX1 载体载体序列
LOCUS Exported 2999 bp ds-DNA circular SYN 18-SEP-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pVAX1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2999)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..2999
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 36..415
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 416..619
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 664..682
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 829..1053
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
CDS 1226..2020
/codon_start=1
/gene="aph(3)-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin 2346..2934
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
