pacAd5 K-NpA shuttle vector does not contain a promoter ahead of the multiple cloning sites. You must clone a promoter into the vector along with your gene of interest.
| 载体基本信息 | |||
| 载体名称 | pacAd5 CMVK-NpA | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 6215 bp | 载体类型 | Adenoviral Expression Vector |
| 载体来源 | Cell BioLabs | 拷贝数 | High copy number |
| 启动子 | CMV | 克隆方法 | Enzyme Cut |
pacAd5 CMVK-NpA 载体载体图谱
pacAd5 CMVK-NpA 载体载体序列
LOCUS Exported 6215 bp ds-DNA circular SYN 17-NOV-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6215)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6215
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 16..118
/note="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 206..356
/note="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
enhancer 385..688
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 689..892
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 928..944
/note="KS primer"
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(978..994)
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 1014..1148
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 1441..3895
/note="3328-5792 of Ad5"
rep_origin complement(4388..4976)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5147..6007)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6008..6112)
/gene="bla"
/note="AmpR promoter"
