Plasmid DNA pMV261 is a replicative vector (multicopy, extrachromosomal) that contains a DNA cassette encoding kanamycin resistance (Tn903-derived aph gene), an E. coli origin of replication (oriE), a mycobacterial plasmid DNA origin of replication (oriM), an expression cassette containing a mycobacterial promoter, a multiple cloning site, and a transcriptional terminator.
| 载体基本信息 | |||
| 载体名称 | pMV261 | 载体抗性 | Kanamycin |
|---|---|---|---|
| 载体长度 | 4488 bp | 载体类型 | Mycobacterium Expression Vector |
| 载体来源 | Stover et al., 1991 Nature 351:456 | 拷贝数 | High copy number |
| 启动子 | Hsp60 | 克隆方法 | Enzyme Cut |
pMV261 载体载体图谱
pMV261 载体载体序列
LOCUS Exported 4488 bp ds-DNA circular SYN 15-DEC-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMV261
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4488)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4488
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 121..936
/codon_start=1
/gene="aph(3)-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1268..1856
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 2015..3910
/note="Mycobacterial OriM region derived from pAL5000"
terminator 4406..4452
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
