The PiggyBac (PB) transposon is a mobile genetic element that efficiently transposes between vectors andchromosomes via a "cut and paste" mechanism. During transposition, the PB transposase recognizes transposonspecific inverted terminal repeat sequences (ITRs) located on both ends of the transposon vector and efficiently moves the contents from the original sites and efficiently integrates them into TTAA chromosomal sites. The powerful activity of the piggyBac transposon system enables genes of interest between the two ITRs in the PB vector to be easily mobilized into target genomes. The Super PiggyBac transposase expression vector features a 5’ HS4 insulator to support robust transcription from the rPolr2A promoter. The PiggyBac transposase coding sequence has been optimized for high expression, stability and activity in mammalian cells.
The sequencing results indicated the XhoI site was destroyed by the insertion GTCGACGCCGCT.
| 载体基本信息 | |||
| 载体名称 | Super PiggyBac Transposase | 载体抗性 | Ampicillin |
|---|---|---|---|
| 载体长度 | 8531 bp | 载体类型 | PiggyBac |
| 载体来源 | SBI | 启动子 | rPolr2A |
Super PiggyBac Transposase 载体载体图谱
Super PiggyBac Transposase 载体载体序列
LOCUS Exported 8531 bp ds-DNA circular SYN 24-DEC-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Super PiggyBac Transposase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8531)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8531
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 2449..2465
/note="SK primer"
/note="common sequencing primer, one of multiple similar
variants"
intron 3442..3538
/note="SV40 intron"
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 3614..5398
/codon_start=1
/note="Super PiggyBac Transposase"
/translation="MGSSLDDEHILSALLQSDDELVGEDSDSEVSDHVSEDDVQSDTEE
AFIDEVHEVQPTSSGSEILDEQNVIEQPGSSLASNRILTLPQRTIRGKNKHCWSTSKST
RRSRVSALNIVRSQRGPTRMCRNIYDPLLCFKLFFTDEIISEIVKWTNAEISLKRRESM
TSATFRDTNEDEIYAFFGILVMTAVRKDNHMSTDDLFDRSLSMVYVSVMSRDRFDFLIR
CLRMDDKSIRPTLRENDVFTPVRKIWDLFIHQCIQNYTPGAHLTIDEQLLGFRGRCPFR
VYIPNKPSKYGIKILMMCDSGTKYMINGMPYLGRGTQTNGVPLGEYYVKELSKPVHGSC
RNITCDNWFTSIPLAKNLLQEPYKLTIVGTVRSNKREIPEVLKNSRSRPVGTSMFCFDG
PLTLVSYKPKPAKMVYLLSSCDEDASINESTGKPQMVMYYNQTKGGVDTLDQMCSVMTC
SRKTNRWPMALLYGMINIACINSFIIYSHNVSSKGEKVQSRKKFMRNLYMSLTSSFMRK
RLEAPTLKRYLRDNISNILPKEVPGTSDDSTEEPVMKKRTYCTYCPSKIRRKANASCKK
CKKVICREHNIDMCQSCF"
polyA_signal 5424..5558
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(5675..5693)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(5714..5730)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 5738..5754
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5762..5792)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5807..5828
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6116..6704)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6875..7735)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7736..7840)
/gene="bla"
/note="AmpR promoter"
rep_origin 7867..8322
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 8463..8479
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 8489..8507
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
