pMG36e plasmid (pWV01 ori) is based on protease gene transcription and translation signals from Lactococcus lactis cremoris, is a typical artificial constitutive expression vector as a main expression system in Lactococcus.It contains a strong promoter and can express foreign proteins in a variety of bacteria. It has been used to study the action mechanism of bacteriocin, the transformation of lactic acid bacteria genetic engineering strains and the development of oral vaccines. It has been widely used in many fields and has become one of the important tools for lactic acid bacteria genetic engineering research.
| 载体基本信息 | |||
| 载体名称 | pMG36e | 载体抗性 | Erythromycin |
|---|---|---|---|
| 载体长度 | 3610 bp | 载体类型 | Lactococcus lactis expression plasmid |
| 拷贝数 | High copy number | 启动子 | P32 |
| 克隆方法 | Enzyme Cut | ||
pMG36e 载体载体图谱
pMG36e 载体载体序列
LOCUS Exported 3610 bp ds-DNA circular SYN 17-JUN-2022
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pMG36e
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3610)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3610
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 227..925
/codon_start=1
/note="repA"
/translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
VLDAETGEIK"
CDS complement(1463..2197)
/codon_start=1
/note="Emr"
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
DLNNISFEQFLSLFNSYKLFNK"
promoter 2474..2652
/note="P32"
CDS 2653..2892
/codon_start=1
/note="ORF"
/translation="MAIVSAEKFVIRARPGIDPLESTCRHASLQSLKTKVAAAVEAAKT
VGKGDGTTGTSDKGGGQGTPALRYLELRFKVKWY"
