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规格:
货期:
编号:TC1001977
品牌:Testobio

产品信息

名称NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)
别称H146; H-146; NCIH146
种属人类
生长特性悬浮,聚团生长
细胞形态淋巴母细胞样
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO                                                    温度:液氮                                                
培养方案A(默认)

生长培养基:

RPMI-1640+10% FBS+1% P/S                                                        

培养条件:

气相:空气,95%;CO2,5%,                                                            温度:37℃                                                        

推荐传代比例1:2-1:3
推荐换液频率2-3次/周
注意事项该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。

参考资料

背景描述Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected.
年龄(性别)男性;59岁
组织来源肺
细胞类型肿瘤细胞
肿瘤类型肺癌细胞
基因表达情况vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -
保藏机构ATCC; HTB-173
首页 > 产品中心 > 细胞生物学 > 细胞培养 > 细胞系 > NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)

NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)

  • 货号: TC1001977
  • 好评
询价
  • 组织来源:肺
  • 品牌 : TESTOBIO

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NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确) ¥1500
NCI-H146 [H146]细胞专用培养基
  • ¥350
  • ¥500

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套餐组合价: ¥1850 ¥2000

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产品信息

名称NCI-H146 [H146] (人小细胞肺癌细胞) (STR鉴定正确)
别称H146; H-146; NCIH146
种属人类
生长特性悬浮,聚团生长
细胞形态淋巴母细胞样
冻存条件冻存液:55% 基础培养基+40%FBS+5%DMSO                                                    温度:液氮                                                
培养方案A(默认)

生长培养基:

RPMI-1640+10% FBS+1% P/S                                                        

培养条件:

气相:空气,95%;CO2,5%,                                                            温度:37℃                                                        

推荐传代比例1:2-1:3
推荐换液频率2-3次/周
注意事项该细胞为悬浮细胞,请注意离心收集细胞悬液;请勿直接倒掉细胞培养液。

参考资料

背景描述Derived from the pleural fluid of a patient with small cell lung cancer; the cells express elevated levels of the 4 biochemical markers characteristic of SCLC: neuron-specific enolase, the brain isoenzyme of creatine kinase, L-dopa decarboxylase and bombesin-like immunoreactivity; the cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein; the cells express relatively high amounts of c-myc mRNA, but no amplification of the gene is detected.
年龄(性别)男性;59岁
组织来源肺
细胞类型肿瘤细胞
肿瘤类型肺癌细胞
基因表达情况vasopressin negative; oxytocin negative; gastrin releasing peptide negative; vimentin positive by immunostaining; keratin positive by immunostaining; neurofilament triplet protein negative; c-myb mRNA; v-fms mRNA; Ha-ras mRNA; Ki-ras mRNA; N-ras mRNA; c-raf 1 mRNA; myc+; myb+; raf+; ras+; fms+; fes -
保藏机构ATCC; HTB-173
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