产品信息
| 名称 | HCC1954 (人乳腺导管癌细胞) (STR鉴定正确) |
| 别称 | HCC-1954; Hamon Cancer Center 1954 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样,有空泡 |
| 冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
| 培养方案A(默认) | 生长培养基: 培养条件: 气相:空气,95%;CO2,5%, 温度:37℃ |
| 推荐传代比例 | 1:4-1:8 |
| 推荐换液频率 | 2-3次/周 |
参考资料
| 背景描述 | HCC1954 is an epithelial cell line isolated from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases from a 61-year-old, Asian female in 1995. HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay. |
| 年龄(性别) | 女性;61岁 |
| 组织来源 | Breast; Duct; Mammary gland |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 乳腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 45 hours (PubMed=25984343); 43.81 hours (GrayJW panel) |
| 受体表达情况 | Estrogen receptor, not expressed; progesterone receptor, not expressed |
| 基因表达情况 | epithelial glycoprotein 2 (EGP2); cytokeratin 19 |
| 保藏机构 | ATCC; CRL-2338 BCRJ; 0280 KCLB; 9S1954 |
产品信息
| 名称 | HCC1954 (人乳腺导管癌细胞) (STR鉴定正确) |
| 别称 | HCC-1954; Hamon Cancer Center 1954 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样,有空泡 |
| 冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
| 培养方案A(默认) | 生长培养基: 培养条件: 气相:空气,95%;CO2,5%, 温度:37℃ |
| 推荐传代比例 | 1:4-1:8 |
| 推荐换液频率 | 2-3次/周 |
参考资料
| 背景描述 | HCC1954 is an epithelial cell line isolated from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases from a 61-year-old, Asian female in 1995. HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay. |
| 年龄(性别) | 女性;61岁 |
| 组织来源 | Breast; Duct; Mammary gland |
| 细胞类型 | 肿瘤细胞 |
| 肿瘤类型 | 乳腺癌细胞 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | 45 hours (PubMed=25984343); 43.81 hours (GrayJW panel) |
| 受体表达情况 | Estrogen receptor, not expressed; progesterone receptor, not expressed |
| 基因表达情况 | epithelial glycoprotein 2 (EGP2); cytokeratin 19 |
| 保藏机构 | ATCC; CRL-2338 BCRJ; 0280 KCLB; 9S1954 |