产品信息
| 名称 | BPH-1 (人前列腺增生细胞) (STR鉴定正确) |
| 别称 | BPH1; Benign Prostatic Hyperplasia-1 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
| 培养方案A(默认) | 生长培养基: RPMI-1640+20%FBS+20 ng/mL testosterone + 0.5% ITS-G(100×)+1% P/S 培养条件: 气相:空气,95%;CO2,5%, 温度:37℃ |
| 推荐传代比例 | 1:3-1:5 |
| 推荐换液频率 | 2-3次/周 |
参考资料
| 背景描述 | prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens. |
| 年龄(性别) | 男性;68岁 |
| 组织来源 | 前列腺 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~50 hours (DSMZ=ACC-143) |
| 保藏机构 | DSMZ; ACC-143 |
产品信息
| 名称 | BPH-1 (人前列腺增生细胞) (STR鉴定正确) |
| 别称 | BPH1; Benign Prostatic Hyperplasia-1 |
| 种属 | 人 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 上皮细胞样 |
| 冻存条件 | 冻存液:55% 基础培养基+40%FBS+5%DMSO 温度:液氮 |
| 培养方案A(默认) | 生长培养基: RPMI-1640+20%FBS+20 ng/mL testosterone + 0.5% ITS-G(100×)+1% P/S 培养条件: 气相:空气,95%;CO2,5%, 温度:37℃ |
| 推荐传代比例 | 1:3-1:5 |
| 推荐换液频率 | 2-3次/周 |
参考资料
| 背景描述 | prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens. |
| 年龄(性别) | 男性;68岁 |
| 组织来源 | 前列腺 |
| 细胞类型 | 转化细胞系 |
| 生物安全等级 | BSL-1 |
| 倍增时间 | ~50 hours (DSMZ=ACC-143) |
| 保藏机构 | DSMZ; ACC-143 |